The β-Catenin antibody (MSVA-578R) is a recombinant rabbit monoclonal antibody optimized for immunohistochemical (IHC) detection of Catenin beta-1 (CTNNB1) in human tissues. Under physiological conditions, β-Catenin is predominantly localized at the cell membrane, where it associates with cadherins to regulate cell–cell adhesion, with additional cytoplasmic and nuclear localization reflecting activation of the Wnt/β-Catenin signaling pathway.
This antibody delivers clear membranous staining in epithelial tissues, with distinct nuclear positivity in a subset of cells, enabling reliable assessment of β-Catenin localization patterns in both normal and tumor tissues.
Product Highlights
Target: β-Catenin (CTNNB1, Catenin beta-1)
Localization: Predominantly cell membrane; cytoplasmic and nuclear in activated states
Application: IHC (FFPE)
Recommended dilution: 1:100–1:200
Host: Recombinant rabbit monoclonal
Reactivity: Human
Expression Pattern (IHC Summary)
Caput epididymis: Strong membranous staining in epithelial cells; focal nuclear positivity
Placenta (mature): Strong staining in cytotrophoblast; negative in syncytiotrophoblast
Thyroid & parathyroid: Strong membranous staining of epithelial cells
Adrenal gland: Moderate membranous staining with focal nuclear and cytoplasmic positivity
Brain: Weak to moderate staining of neural structures
Cancer Research Relevance
Most epithelial tumors show membranous β-Catenin expression, while a subset displays cytoplasmic and nuclear accumulation, reflecting Wnt pathway dysregulation. Nuclear β-Catenin staining is frequently observed in cancers such as colorectal, esophageal, and cervical carcinomas, making this antibody valuable for Wnt/β-Catenin pathway research and tumor biology studies.
Validation
MSVA-578R has been validated using an orthogonal strategy, showing strong concordance between IHC staining patterns and RNA expression data from Human Protein Atlas (HPA), FANTOM5, and GTEx datasets.
Research Use Only
For research use only. Not intended for diagnostic or therapeutic applications.
