The Brachyury antibody (HMV321) is a recombinant rabbit monoclonal antibody designed for immunohistochemical (IHC) detection of Brachyury (T-box transcription factor T / TBXT) in human tissues. Brachyury is a nuclear transcription factor pivotal for mesoderm development and posterior body formation during embryogenesis. In adults, Brachyury expression is absent in normal tissues, making it a highly specific marker for chordoma research.
This antibody provides robust nuclear staining in chordoma tissues, with high sensitivity for conventional chordomas, while being consistently negative in normal kidney, brain, endocrine, respiratory, gastrointestinal, and reproductive tissues.
Target: Brachyury (TBXT, T-box transcription factor T)
Localization: Nucleus
Application: IHC (FFPE)
Recommended dilution: 1:100–1:200
Host: Recombinant rabbit monoclonal
Reactivity: Human
Expression & Research Relevance
Normal tissues: Completely negative, including kidney, brain, endocrine organs, and reproductive tissues
Chordoma: Strong nuclear positivity in the majority of tumor cells
Other tumors: Expression may be seen in embryonal carcinoma; absent in chondrosarcoma
Clinical significance: Brachyury is a highly specific marker for chordoma, and its absence in normal tissues suggests potential as a therapeutic target
Validation
HMV321 specificity is supported by orthogonal validation, showing complete concordance between IHC staining patterns and RNA expression data from Human Protein Atlas (HPA), FANTOM5, and GTEx datasets.
Research Use Only
For research use only. Not intended for diagnostic or therapeutic applications.
