The BRD4 antibody (HMV4275) is a recombinant rabbit monoclonal antibody designed for immunohistochemical (IHC) detection of Bromodomain-containing protein 4 (BRD4) in human tissues. BRD4 is a nuclear epigenetic regulator and member of the BET protein family, involved in chromatin organization, transcriptional regulation, and interaction with key oncogenic proteins such as c-MYC. It is widely expressed across normal tissues and serves as a highly investigated therapeutic target.
This antibody produces distinct nuclear staining in epithelial and stromal cells, enabling robust detection of BRD4 in normal and tumor tissues.
Product Highlights
Target: BRD4 (Bromodomain-containing protein 4, CAP, FSHRG4, HUNK1)
Localization: Nucleus
Application: IHC (FFPE)
Recommended dilution: 1:100–1:200
Host: Recombinant rabbit monoclonal
Reactivity: Human
Expression & Research Relevance
Normal tissues: Strong nuclear staining in kidney, thyroid, parathyroid, adrenal gland, respiratory epithelium, lung, and glandular tissues; variable intensity in brain glia and cerebellar neurons
Cancer tissues: BRD4 is expressed in a wide range of epithelial tumors. Staining intensity may vary, with some tumors showing strong nuclear positivity, while others have weak or focal expression
Research focus: BRD4 is widely studied as a therapeutic target in oncology and epigenetic regulation
Validation
HMV4275 specificity is confirmed via orthogonal validation, consistent with RNA expression data from Human Protein Atlas (HPA), FANTOM5, and GTEx datasets.
Research Use Only
For research use only. Not intended for diagnostic or therapeutic applications.
