TRPS1 (MSVA-512R)

Rabbit monoclonal / IgG 1:100 – 1:200 Research Use Only Nucleus Human MSVA-512R GC79; LGCR; Transcriptional repressor GATA binding 1; Tricho-rhino-phalangeal syndrome type I protein; Trichorhinophalangeal syndrome I; Trichorhinophalangeal syndrome I homolog; TRPS 1; trpS1; TRPS1 gene; TRPS1_HUMAN; Zinc finger protein GC79; Zinc finger transcription factor TRPS 1 antibody Breast: A strong TRPS1 staining should be seen in luminal breast epithelial cells. (For low-level TRPS1 detection: Testis: A weak nuclear TRPS1 staining should be seen in spermatogonia.) Colon: Nuclear TRPS1 staining should be absent in epithelial cells. TRPS1 is a Transcription factor highly expressed in breast epithelial tissues. TRPS1 ( Transcriptional Repressor GATA Binding 1) is a nuclear transcription factor protein coded by the TRPS1 gene on chromosome 8q23-24. In contrast to other GATA-type transcription factors, TRPS1 mainly acts as a transcriptional repressor. TRPS1 represses GATA-regulated genes by binding to the dynein light chain protein. TRPS1-dynein binding hinders dynein-GATA binding and thus suppresses its transcriptional activity. For example, TRPS1 can directly suppress the expression of Runx1 and Sox9 in cartilage formation. Several hereditary TRPS1 mutations are known to result in craniofacial and skeletal malformations. TRPS1 can also induce expression of genes such as for example FOXA1, a negative regulator of epithelial mesenchymal transition (EMT). It was suggested that TRPS1 has tumor-suppressive activity by preventing EMT. Aberrant expression of TRPS1 occurs in various types of cancers. Images describing the TRPS1 staining pattern in normal tissues obtained by the antibody MSVA- 512R are shown in our “ Normal Tissue Gallery ”. Brain Cerebrum Moderate nuclear TRPS1 staining of glia cells. Cerebellum Moderate nuclear TRPS1 staining of glia cells. Endocrine Tissues Thyroid Faint nuclear TRPS1 staining of a fraction of epithelial cells. Parathyroid Weak to moderate nuclear TRPS1 staining of epithelial cells. Adrenal gland Negative. Pituitary gland Faint nuclear TRPS1 staining of a fraction of epithelial cells. Respiratory system Respiratory epithelium Weak to moderate nuclear TRPS1 staining of a fraction of respiratory epithelial cells and of some glandular cells from bronchus glands. Lung Negative. Gastrointestinal Tract Salivary glands Weak nuclear TRPS1 staining in glandular (serous and mucinous) and excretory duct cells. Esophagus Weak to moderate nuclear TRPS1 staining of suprabasal squamous epithelial cells. Stomach Epithelial cells are TRPS1 negative. Duodenum Epit... A strong TRPS1 immunostaining preferentially occurs in breast cancer. TRPS1 positivity – usually at a lower level of intensity – also occurs in many other tumor entities derived from the female genital tract and from various other organs. If TRPS1 should be used as a marker for breast cancer, a much higher dilution is recommended than for use for low-level TRPS1 detection. The TCGA findings on TRPS1 RNA expression in different tumor categories have been summarized in the Human Protein Atlas. Breast – Invasive breast cancer of no special type (NST) with strong TRPS1 staining of all tumor cells (MSVA-512R, diluted at 1-600) Urinary bladder –TRPS1 negative muscle-invasive urothelial carcinoma (MSVA-512R, diluted at 1-600) Ovary – Serous high-grade carcinoma with strong TRPS1 staining of tumor cells (MSVA-512R, diluted at 1-600) Cancer tissue gallery TRPS1 (MSVA-512R) publication summary Relevant publication: Lennartz et al. “TRPS1 is a highly sensitive marker for breast cancer: A tissue microarray study evaluating more than 19,000 tumors from 152 different tumor entities” Published in Am J Surg Pathol. 2024 Apr 22. doi: 10.1097/PAS.0000000000002213. Epub ahead of print. PMID: 38647255. A total of 16,818 tumors from 152 different tumor categories were successfully analyzed by using the following protocol: Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 9,0 Target Retrieval Solution buffer. MSVA-512R, at a dilution of 1:300 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent). This protocol was also used for all stainings depicted in our tumor and normal tissue galleries. Overall, 86 of 152 tumor categories showed detectable TRPS1 staining with 36 tumor categories showing at least one strongly positive case. The TRPS1 positivity rate was highest in various types of b... IHC users have different preferences on how the stains should look like. Some prefer high staining intensity of the target stain and even accept some background. Others favor absolute specificity and lighter target stains. Factors that invariably lead to more intense staining include higher concentration of the antibody and visualization tools, longer incubation time, higher temperature during incubation, higher temperature and longer duration of the heat induced epitope retrieval (slide pretreatment). The impact of the pH during slide pretreatment has variable effects and depends on the antibody and the target protein. All images and data shown here and in our image galleries are obtained by the manual protocol described below. Other protocols resulting in equivalent staining are described as well. Manual protocol Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target ... The role of TRPS1 in the biology of breast cancer and other tumors needs to be investigated. The role of TRPS1 in epithelial mesenchymal transition is under investigation. The diagnostic utility of TRPS1 immunohistochemistry awaits further clarification. There are two ways how the specificity of antibodies can be documented for immunohistochemistry on formalin fixed tissues. These are: 1. Comparison with a second independent method for target expression measurement across a large number of different tissue types (orthogonal strategy), and 2. Comparison with one or several independent antibodies for the same target and showing that all positive staining results are also seen with other antibodies for the same target (independent antibody strategy). Orthogonal validation: For the antibody MSVA- 512R, a comparison with RNA data with data from three independent RNA screening studies, including the Human Protein Atlas (HPA) RNA-seq tissue dataset, the FANTOM5 project, and the Genotype-Tissue Expression (GTEx) project, which are all summarized in the Human Protein Atlas (Tissue expression TRPS1) is consistent with a specific staining as immunostaining by MSVA- 512R . At least TRPS1 immunostaining was strongest in the breast (the organ with h...